Journal: Clinical Science (London, England : 1979)

Article Title: Transcriptomic and functional analysis of fibroid extracellular vesicles

doi: 10.1042/CS20250814

Figure Lengend Snippet: Fib-EXO enhance angiogenic activity in HUVECs. ( A ) Representative fluorescence images of HUVECs seeded on Matrigel and exposed to ( a ) medium-derived EVs (Control-EXO), ( b ) Fib-EXO, or ( c ) Fib-EXO in the presence of the EV uptake inhibitor Y27632 (10 μM; Fib-EXO/Y27632). Cells were stained using the reagent provided in the angiogenesis assay kit. Quantitative analysis of tube formation shows that HUVECs treated with Fib-EXO exhibited significantly increased total tube length ( B ) and total branching points ( C ) relative to Control-EXO or inhibitor-treated samples. Data are presented as P <0.01 (**).

Article Snippet: Endothelial tube formation was assessed using a commercial angiogenesis assay kit (Cell Biolabs Inc., San Diego, CA) in accordance with the manufacturer’s instructions.

Techniques: Activity Assay, Fluorescence, Derivative Assay, Control, Staining, Angiogenesis Assay

Journal: Cells

Article Title: Extracellular Vesicles Derived from VEGF mRNA-Engineered Mesenchymal Stem Cells Promote Endothelial Cell Survival

doi: 10.3390/cells15080717

Figure Lengend Snippet: Angiogenesis induced by VEGF secreted from VEGF-MSCs. Human umbilical vein endothelial cells labelled by CytoLight Green (GFP-HUVECs) were used for the development of angiogenic networks, and the length of networks was determined by the IncuCyte live-cell analysis system. ( A ) VEGF effectively induced the formation of vascular networks, which was able to be inhibited by suramin, a VEGF signaling inhibitor. Data shown as mean (SD) ( n = 6). ( B ) A concentration-dependent effect was shown in the angiogenesis assay using CCM-VEGF with different VEGF concentrations (1, 2, 4, and 8 ng/mL). Blank control, without any VEGF supplement added; suramin group, 4 ng/mL VEGF and 100 µM suramin added. Data shown as mean (SD) ( n = 4); ns, not significant; **** p < 0.05.

Article Snippet: IncuCyte Angiogenesis 96-well PrimeKit (Essen Bioscience, Ann Arbor, MI, USA, catalog# 4452) and IncuCyte Angiogenesis PrimeKit VEGF/Suramin Supplement Kit (Essen Bioscience, catalog# 4437) were used as per the manufacturer’s protocols with some modifications.

Techniques: Cell Analysis, Concentration Assay, Angiogenesis Assay, Control

Journal: Cells

Article Title: Extracellular Vesicles Derived from VEGF mRNA-Engineered Mesenchymal Stem Cells Promote Endothelial Cell Survival

doi: 10.3390/cells15080717

Figure Lengend Snippet: Angiogenesis induced by VEGF secreted from VEGF-MSCs. Human umbilical vein endothelial cells labelled by CytoLight Green (GFP-HUVECs) were used for the development of angiogenic networks, and the length of networks was determined by the IncuCyte live-cell analysis system. ( A ) VEGF effectively induced the formation of vascular networks, which was able to be inhibited by suramin, a VEGF signaling inhibitor. Data shown as mean (SD) ( n = 6). ( B ) A concentration-dependent effect was shown in the angiogenesis assay using CCM-VEGF with different VEGF concentrations (1, 2, 4, and 8 ng/mL). Blank control, without any VEGF supplement added; suramin group, 4 ng/mL VEGF and 100 µM suramin added. Data shown as mean (SD) ( n = 4); ns, not significant; **** p < 0.05.

Article Snippet: Image analysis was performed using the IncuCyte automated angiogenesis algorithm (Incucyte software, v2024B, Sartorius).

Techniques: Cell Analysis, Concentration Assay, Angiogenesis Assay, Control